Isolation and identification of restriction endonuclease BstF I
نویسندگان
چکیده
منابع مشابه
Isolation and identification of restriction endonuclease BsiSI.
BseBI, an isoschizomer of BstNI (1) has been purified from Bacillus stearothermopilus species. BseBI recognises the sequence 5'...CCWGG...3' (W=A or T) and cleaves between C and W. The enzyme was purified using the following Chromatographic steps: 1. Blue -Sepharose F3GA, 2. Heparin -Sepharose, 3. DEAE-Sepharose. The enzyme was free of contaminating nuclease activity. After 5-fold overdigestion...
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SseBI, an isoschizomer of StuI (1) has been purified from Streptomyces species. SseBI recognises the sequence 5' ... AGGCCT ... 3' and cleaves between G and C. The enzyme was purified using the following chromatographic steps: 1. Blue Sepharose F3GA, 2. Heparin-Sepharose. The enzyme was free of contaminating nuclease activity. After 100 fold overdigestion on lambda DNA greater than 95% of the D...
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Ptal is a type II restriction endonuclease from the Cyanobacterial strain Phormidium tadzschicicum. This strain was obtained from the Collection of Algal Cultures of Leningrad University. Ptal recognizes the sequence 5'-TCCGGA-3' and cleaves between T and C. The enzyme was purified using the following chromatographic steps: 1) Phosphocelulose, 2) DEAESephadex. The enzyme was free of contaminati...
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Asp35Hl, a type II restriction endonuclease, has been isolated from Acidiphilium species 35H. As/?35HI, an isoschizomer of Bsml (1), recognizes the six base non-palindromic sequence 5'GAATGC3', and cleaves one nucleotide outside of the recognition sequence on one strand, and within the recognition sequence on the opposite strand, to generate a two base 3' overhang. The enzyme was purified using...
متن کاملRestriction endonuclease from thermophilic bacterial species III. Isolation and characterization of BsiHKA I.
BsiHKA I is a type II restriction endonuclease from a Bacillus stearothermophilus strain isolated from soil according to (1). fisiHKA I in 11 culture (4.7 g wet cells) was purified by a DEAEsephacel column (30 ml bed volume). Enzyme eluted at about 0.3 M NaCl was dialysed against buffer A (1) and loaded onto a heparin column (8 ml bed volume). Enzyme eluted at 0.4—0.5 M NaCl was dialysed agains...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.17.7205